PA5-31029

antibody from Invitrogen Antibodies

Targeting: SIRT5

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-31029

Provider

Invitrogen Antibodies

Product name

SIRT5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Predicted reactivity: Mouse (90%), Rat (89%), Xenopus laevis (81%), Dog (90%), Pig (89%), Chicken (82%), Bovine (90%).

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using SIRT5 Polyclonal Antibody (Product # PA5-31029). HCT116 whole cell and membrane extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with SIRT5 Polyclonal Antibody (Product # PA5-31029) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using SIRT5 Polyclonal Antibody (Product # PA5-31029). Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with SIRT5 Polyclonal Antibody (Product # PA5-31029) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human hepatoma, using SIRT5 (Product # PA5-31029) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.