MA5-55336

antibody from Invitrogen Antibodies

Targeting: SIRT5

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

MA5-55336

Provider

Invitrogen Antibodies

Product name

SIRT5 Recombinant Rabbit Monoclonal Antibody (2U8A2)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Immunogen Sequence: SSSMADFRKF FAKAKHIVII SGAGVSAESG VPTFRGAGGY WRKWQAQDLA TPLAFAHNPS RVWEFYHYRR EVMGSKEPNA GHRAIAECET RLGKQGRRVV VITQNIDELH RKAGTKNLLE IHGSLFKTRC TSCGVVAENY KSPICPALSG KGAPEPGTQD ASIPVEKLPR CEEAGCGGLL RPHVVWFGEN LDPAILEEVD RELAHCDLCL VVGTSSVVYP AAMFAPQVAA RGVPVAEFNT ETTPATNRFR FHFQGPCGTT LPEALACHEN ETVS

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

2U8A2

Vial size

100 μL

Concentration

1.89 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of various lysates using SIRT5 Recombinant Monoclonal Antibody (Product # MA5-55336) at 1:3,000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of various lysates using SIRT5 Recombinant Monoclonal Antibody (Product # MA5-55336) at 1:3,000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.