Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [5]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-109668 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DUSP8 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Immunogen sequence: NleAGDRLPR KVNl
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.97 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DUSP8 in rat lung. Samples were incubated with DUSP8 Polyclonal antibody (Product # PA5-109668) using a dilution of 1:2,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of DUSP8 in L929 cells. Samples were incubated in polyclonal DUSP8 antibody (Product # PA5-109668) using a dilution of 1:100, followed by DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of DUSP8 in HeLa cells. Samples were incubated with DUSP8 Polyclonal antibody (Product # PA5-109668) using a dilution of 1:1,000 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of DUSP8 in MCF7 cells. Samples were incubated with DUSP8 Polyclonal antibody (Product # PA5-109668) using a dilution of 1:1,000 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of DUSP8 in NIH/3T3 cells. Samples were incubated with DUSP8 Polyclonal antibody (Product # PA5-109668) using a dilution of 1:1,000 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of DUSP8 in PC-12 cells. Samples were incubated with DUSP8 Polyclonal antibody (Product # PA5-109668) using a dilution of 1:1,000 (40x lens). Blue: DAPI for nuclear staining.
