LS-C288703

antibody from LSBio

Targeting: CCNK CPR4

Western blot (Enhanced validation)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C288703

Provider

LSBio

Product name

CCNK Antibody (aa530-580) LS-C288703

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store at 2-8°C for up to one year.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of Human Cyclin K by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-Cyclin K antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of human Cyclin K by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cyclin K antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of Human K by Immunohistochemistry. Sample: FFPE sections of human testicular seminoma (left), breast carcinoma (center) and ovarian carcinoma (right). Antibody: Affinity purified rabbit anti-K used at a dilution of 1:1000 (1 Detection: DAB.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of human Cyclin K by immunohistochemistry. Sample: FFPE section of human head and neck squamous cell carcinoma. Antibody: Affinity purified rabbit anti-Cyclin K used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB