Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NBP1-06519 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-06519, RRID:AB_1582356
- Product name
- Rabbit Polyclonal CCNK Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Ovarian carcinoma CDK12 mutations misregulate expression of DNA repair genes via deficient formation and function of the Cdk12/CycK complex.
Ekumi KM, Paculova H, Lenasi T, Pospichalova V, Bösken CA, Rybarikova J, Bryja V, Geyer M, Blazek D, Barboric M
Nucleic acids research 2015 Mar 11;43(5):2575-89
Nucleic acids research 2015 Mar 11;43(5):2575-89
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CCNK Antibody [NBP1-06519] - Whole cell lysate from HeLa cells. PDZ-RhoGEF was also immunoprecipitated by rabbit anti-PDZ-RhoGEF antibody NBP1-06518.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CCNK Antibody [NBP1-06519] - Detection of Human Cyclin K by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cyclin K antibody NBP1-06519 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CCNK Antibody [NBP1-06519] - Whole cell lysate (50 ug) from HEK293T, MCF-7, Jurkat, HeLa, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiCyclin K antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CCNK Antibody [NBP1-06519] - Detection of human Cyclin K by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Cyclin K antibody NBP1-06519 (lot NBP1-06519-6) used for IP at 6 ug per reaction. Cyclin K was also immunoprecipitated by a previous lot of this antibody (lot NBP1-06519-5) and another rabbit anti-Cyclin K antibody. For blotting immunoprecipitated Cyclin K, NBP1-06519 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CCNK Antibody [NBP1-06519] - FFPE section of human head and neck squamous cell carcinoma. Antibody: Affinity purified rabbit antiCyclin K used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CCNK Antibody [NBP1-06519] - Section of human head and neck squamous cell carcinoma. Antibody: Affinity purified rabbit antiCyclin K used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB