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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA5-65600 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NPNT Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: TVDNRVQTDPQ KPRGDVFIPR QPSNDLFEIF EIERGVSADD EAKDDPGVLV HSCNFDHGLC GWIREKDNDL HWEPIRDPAG GQYLTVSAAK APGGKAARLV LPLGRLMHSG DLCLSFRHKV TGLHSGTLQV FVRKHGAHGA ALWGRNGGHG WRQTQITL
- Concentration
- 0.05 mg/mL
Submitted references BRG1 Mediates Nephronectin Activation in Hepatocytes to Promote T Lymphocyte Infiltration in ConA-Induced Hepatitis.
Hong W, Kong M, Qi M, Bai H, Fan Z, Zhang Z, Sun A, Fan X, Xu Y
Frontiers in cell and developmental biology 2020;8:587502
Frontiers in cell and developmental biology 2020;8:587502
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NPNT in human cell line RT-4, human cell line U-251 MG, human plasma and human liver tissue. Samples were probed using a NPNT Polyclonal Antibody (Product # PA5-65600).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of NPNT in human cell line MCF7 shows localization to nucleus and cytosol. Samples were probed using a NPNT Polyclonal Antibody (Product # PA5-65600).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 4 BRG1 regulates nephronectin expression in hepatocytes to promote T cell migration. (A-D) HepG2 cells were transfected with siRNA targeting BRG1 or scrambled siRNA (SCR) followed by treatment with ConA. Nephronectin expression was examined by qPCR, Western blotting, and ELISA. T cell migration was evaluated by transwell assay. (E-H) Primary hepatocytes were isolated from BRG1 LKO mice and WT mice and treated with ConA. Nephronectin expression was examined by qPCR, Western blotting, and ELISA. T cell migration was evaluated by transwell assay. Data represent averages of three independent experiments and error bars represent SEM. Student's t -test was used for statistical analyses. * p < 0.05.
