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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- PA5-80429 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD300g Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
- Concentration
- 5 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD300g in Lane A: Jurkat Whole Cell Lysate (30 µg). Samples were probed using a CD300g Polyclonal Antibody (Product # PA5-80429) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:36 kDa. Observed band size:45 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CD300g Polyclonal Antibody (Product # PA5-80429) at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 36 kDa. Observed band size: 45 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CD300g Immunoprecipitation using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 0.5 µL with CD300g Polyclonal Antibody (Product # PA5-80429) and 15 µL of 50 % Protein G agarose. Primary antibody: CD300g Polyclonal Antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 38 kDa.
