Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF3377 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IFITM3/Fragilis Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse IFITM3/Fragilis in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human IFITM3 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references OTX2 restricts entry to the mouse germline.
Influenza virus differentially activates mTORC1 and mTORC2 signaling to maximize late stage replication.
IFITM1 suppresses expression of human endogenous retroviruses in human embryonic stem cells.
Interferon-induced transmembrane protein 3 is a type II transmembrane protein.
Ifitm3 limits the severity of acute influenza in mice.
Zhang J, Zhang M, Acampora D, Vojtek M, Yuan D, Simeone A, Chambers I
Nature 2018 Oct;562(7728):595-599
Nature 2018 Oct;562(7728):595-599
Influenza virus differentially activates mTORC1 and mTORC2 signaling to maximize late stage replication.
Kuss-Duerkop SK, Wang J, Mena I, White K, Metreveli G, Sakthivel R, Mata MA, Muñoz-Moreno R, Chen X, Krammer F, Diamond MS, Chen ZJ, García-Sastre A, Fontoura BMA
PLoS pathogens 2017 Sep;13(9):e1006635
PLoS pathogens 2017 Sep;13(9):e1006635
IFITM1 suppresses expression of human endogenous retroviruses in human embryonic stem cells.
Fu Y, Zhou Z, Wang H, Gong P, Guo R, Wang J, Lu X, Qi F, Liu L
FEBS open bio 2017 Aug;7(8):1102-1110
FEBS open bio 2017 Aug;7(8):1102-1110
Interferon-induced transmembrane protein 3 is a type II transmembrane protein.
Bailey CC, Kondur HR, Huang IC, Farzan M
The Journal of biological chemistry 2013 Nov 8;288(45):32184-93
The Journal of biological chemistry 2013 Nov 8;288(45):32184-93
Ifitm3 limits the severity of acute influenza in mice.
Bailey CC, Huang IC, Kam C, Farzan M
PLoS pathogens 2012 Sep;8(9):e1002909
PLoS pathogens 2012 Sep;8(9):e1002909
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse IFITM3/Fragilis by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, D3 mouse embryonic stem cell line, and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse IFITM3/Fragilis Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3377) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IFITM3/Fragilis at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- IFITM3/Fragilis in Embryonic Mouse Ovary. IFITM3/Fragilis was detected in immersion fixed frozen sections of embryonic mouse ovary (E13.5) using 10 µg/mL Goat Anti-Mouse IFITM3/Fragilis Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3377) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.