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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- TA324408 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal Interferon-inducible protein (IFITM3) Antibody (N-term)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal Interferon-inducible protein (IFITM3) Antibody (N-term)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- IFITM3
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 2.0 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IFITM3 Antibody (N-term) (Cat. #TA324408) western blot analysis in Hela,293,A549,NCI-H460,HepG2,Sk-Br-3,A375 cell line lysates (35ug/lane).This demonstrates the IFITM3 antibody detected the IFITM3 protein (arrow).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of lysate from HT1080 cell line,using IFITM3 Antibody (N-term)(Cat. #TA324408).TA324408 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of HeLa cells stained with IFITM3 (N-term) antibody. HeLa cells were incubated with Fragilis (IFITM3) (N-term) primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin . Nuclei were counterstained with Hoechst 33342 (blue). Note the highly specific localization of the IFITM3 immunoreactivity to the Golgi.
- Validation comment
- IF
