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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
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- Product number
- PAB2360 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2360, RRID:AB_1576173
- Product name
- IFITM3 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of IFITM3.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references IFITM/Mil/fragilis family proteins IFITM1 and IFITM3 play distinct roles in mouse primordial germ cell homing and repulsion.
Tanaka SS, Yamaguchi YL, Tsoi B, Lickert H, Tam PP
Developmental cell 2005 Dec;9(6):745-56
Developmental cell 2005 Dec;9(6):745-56
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of IFITM3 polyclonal antibody (Cat # PAB2360) in 293 cell line lysates (35 ug/lane). IFITM3 (arrow) was detected using the purified polyclonal antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- HeLa cells stained with IFITM3 polyclonal antibody (Cat # PAB2360, 1:250), without (A) or with (B) blocking peptide (P0250, 1:200).
- Validation comment
- Immunofluorescence
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- WI-38 (primary human fibroblasts) treated with IFITM3 polyclonal antibody (Cat # PAB2360)(Red, 1:250).Secondary: Alexafluor 568, 1:1000. Cellular membranes stained with wheat germ agglutinin (orange).Microtubules stained with phalloidin (green).Nuclei stained with DAPI (blue).
- Validation comment
- Immunofluorescence
