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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- F54274 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- IFITM3 Antibody / Interferon-inducible protein
- Antibody type
- Polyclonal
- Description
- This highly specific IFITM3 antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence applications with human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 0.08 ml, 0.4 ml
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the IFITM3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HeLa, 2) 293, 3) A549, 4) NCI-H460, 5) HepG2, 6) SK-B-R3 and 7) A375 cell lysate with IFITM3 antibody. Predicted molecular weight: ~15/17 kDa (unmodified/lipidated).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HeLa, 2) 293, 3) HepG2, 4) NCI-H460, 5) SK-B-R3, 6) placent and 7) mouse colon lysate with IFITM3 antibody. Predicted molecular weight: ~15/17 kDa (unmodified/lipidated).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of fixed and permeabilized human HeLa cells with IFITM3 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human spleen tissue with IFITM3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human colon carcinoma tissue with IFITM3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
