Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [15]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 66081-1-Ig - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#66081-1-Ig, RRID:AB_11182821
- Product name
- IFITM2/3 antibody
- Antibody type
- Monoclonal
- Description
- KD/KO validated IFITM2/3 antibody (Cat. #66081-1-Ig) is a mouse monoclonal antibody that shows reactivity with human and has been validated for the following applications: FC, IHC, WB,ELISA.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 2E8D12
- Vial size
- 20ul, 150ul
Submitted references Omicron Spike confers enhanced infectivity and interferon resistance to SARS-CoV-2 in human nasal tissue.
Cyclosporines Antagonize the Antiviral Activity of IFITMProteins by Redistributing Them toward the Golgi Apparatus.
Single-cell RNA-sequencing uncovers the dynamic changes of tumour immune microenvironment in advanced lung adenocarcinoma.
Inhibition of Arenavirus Entry and Replication by the Cell-Intrinsic Restriction Factor ZMPSTE24 Is Enhanced by IFITM Antiviral Activity.
Escape of HIV-1 envelope glycoprotein from the restriction of infection by IFITM3.
IFITM3 Reduces Retroviral Envelope Abundance and Function and Is Counteracted by glycoGag.
Syncytia formation by SARS-CoV-2-infected cells.
IFITM proteins inhibit placental syncytiotrophoblast formation and promote fetal demise.
Analysis of IFITM-IFITM Interactions by a Flow Cytometry-Based FRET Assay.
Influenza A Virus Facilitates Its Infectivity by Activating p53 to Inhibit the Expression of Interferon-Induced Transmembrane Proteins.
mTOR inhibitors lower an intrinsic barrier to virus infection mediated by IFITM3.
Late Endosomal/Lysosomal Cholesterol Accumulation Is a Host Cell-Protective Mechanism Inhibiting Endosomal Escape of Influenza A Virus.
Interferon-Induced Transmembrane Protein 3 Inhibits Hantaan Virus Infection, and Its Single Nucleotide Polymorphism rs12252 Influences the Severity of Hemorrhagic Fever with Renal Syndrome.
Natural mutations in IFITM3 modulate post-translational regulation and toggle antiviral specificity.
Human cytomegalovirus exploits interferon-induced transmembrane proteins to facilitate morphogenesis of the virion assembly compartment.
Shi G, Li T, Lai KK, Johnson RF, Yewdell JW, Compton AA
Nature communications 2024 Jan 30;15(1):889
Nature communications 2024 Jan 30;15(1):889
Cyclosporines Antagonize the Antiviral Activity of IFITMProteins by Redistributing Them toward the Golgi Apparatus.
Prikryl D, Marin M, Desai TM, Du Y, Fu H, Melikyan GB
Biomolecules 2023 Jun 3;13(6)
Biomolecules 2023 Jun 3;13(6)
Single-cell RNA-sequencing uncovers the dynamic changes of tumour immune microenvironment in advanced lung adenocarcinoma.
Lu H, Qian J, Cheng L, Shen Y, Chu T, Zhao C
BMJ open respiratory research 2023 Dec 11;10(1)
BMJ open respiratory research 2023 Dec 11;10(1)
Inhibition of Arenavirus Entry and Replication by the Cell-Intrinsic Restriction Factor ZMPSTE24 Is Enhanced by IFITM Antiviral Activity.
Stott-Marshall RJ, Foster TL
Frontiers in microbiology 2022;13:840885
Frontiers in microbiology 2022;13:840885
Escape of HIV-1 envelope glycoprotein from the restriction of infection by IFITM3.
Drouin A, Migraine J, Durand MA, Moreau A, Burlaud-Gaillard J, Beretta M, Roingeard P, Bouvin-Pley M, Braibant M
Journal of virology 2021 Mar 1;95(5)
Journal of virology 2021 Mar 1;95(5)
IFITM3 Reduces Retroviral Envelope Abundance and Function and Is Counteracted by glycoGag.
Ahi YS, Yimer D, Shi G, Majdoul S, Rahman K, Rein A, Compton AA
mBio 2020 Jan 21;11(1)
mBio 2020 Jan 21;11(1)
Syncytia formation by SARS-CoV-2-infected cells.
Buchrieser J, Dufloo J, Hubert M, Monel B, Planas D, Rajah MM, Planchais C, Porrot F, Guivel-Benhassine F, Van der Werf S, Casartelli N, Mouquet H, Bruel T, Schwartz O
The EMBO journal 2020 Dec 1;39(23):e106267
The EMBO journal 2020 Dec 1;39(23):e106267
IFITM proteins inhibit placental syncytiotrophoblast formation and promote fetal demise.
Buchrieser J, Degrelle SA, Couderc T, Nevers Q, Disson O, Manet C, Donahue DA, Porrot F, Hillion KH, Perthame E, Arroyo MV, Souquere S, Ruigrok K, Dupressoir A, Heidmann T, Montagutelli X, Fournier T, Lecuit M, Schwartz O
Science (New York, N.Y.) 2019 Jul 12;365(6449):176-180
Science (New York, N.Y.) 2019 Jul 12;365(6449):176-180
Analysis of IFITM-IFITM Interactions by a Flow Cytometry-Based FRET Assay.
Winkler M, Wrensch F, Bosch P, Knoth M, Schindler M, Gärtner S, Pöhlmann S
International journal of molecular sciences 2019 Aug 8;20(16)
International journal of molecular sciences 2019 Aug 8;20(16)
Influenza A Virus Facilitates Its Infectivity by Activating p53 to Inhibit the Expression of Interferon-Induced Transmembrane Proteins.
Wang B, Lam TH, Soh MK, Ye Z, Chen J, Ren EC
Frontiers in immunology 2018;9:1193
Frontiers in immunology 2018;9:1193
mTOR inhibitors lower an intrinsic barrier to virus infection mediated by IFITM3.
Shi G, Ozog S, Torbett BE, Compton AA
Proceedings of the National Academy of Sciences of the United States of America 2018 Oct 23;115(43):E10069-E10078
Proceedings of the National Academy of Sciences of the United States of America 2018 Oct 23;115(43):E10069-E10078
Late Endosomal/Lysosomal Cholesterol Accumulation Is a Host Cell-Protective Mechanism Inhibiting Endosomal Escape of Influenza A Virus.
Kühnl A, Musiol A, Heitzig N, Johnson DE, Ehrhardt C, Grewal T, Gerke V, Ludwig S, Rescher U
mBio 2018 Jul 24;9(4)
mBio 2018 Jul 24;9(4)
Interferon-Induced Transmembrane Protein 3 Inhibits Hantaan Virus Infection, and Its Single Nucleotide Polymorphism rs12252 Influences the Severity of Hemorrhagic Fever with Renal Syndrome.
Xu-Yang Z, Pei-Yu B, Chuan-Tao Y, Wei Y, Hong-Wei M, Kang T, Chun-Mei Z, Ying-Feng L, Xin W, Ping-Zhong W, Chang-Xing H, Xue-Fan B, Ying Z, Zhan-Sheng J
Frontiers in immunology 2016;7:535
Frontiers in immunology 2016;7:535
Natural mutations in IFITM3 modulate post-translational regulation and toggle antiviral specificity.
Compton AA, Roy N, Porrot F, Billet A, Casartelli N, Yount JS, Liang C, Schwartz O
EMBO reports 2016 Nov;17(11):1657-1671
EMBO reports 2016 Nov;17(11):1657-1671
Human cytomegalovirus exploits interferon-induced transmembrane proteins to facilitate morphogenesis of the virion assembly compartment.
Xie M, Xuan B, Shan J, Pan D, Sun Y, Shan Z, Zhang J, Yu D, Li B, Qian Z
Journal of virology 2015 Mar;89(6):3049-61
Journal of virology 2015 Mar;89(6):3049-61
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 66081-1-Ig(IFITM3 antibody) at dilution of 1:1000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using IFITM3 antibody 66081-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human lung cancer using 66081-1-Ig(IFITM3 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
