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LearnNB500-241
antibody from Novus Biologicals
Targeting: TRPM2
EREG1, KNP3, LTRPC2, NUDT9H, NUDT9L1, TRPC7
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- NB500-241 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB500-241, RRID:AB_10001823
- Product name
- Rabbit Polyclonal TRPM2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Novel CaM-binding motif in its NudT9H domain contributes to temperature sensitivity of TRPM2.
Structure-activity relationship of adenosine 5'-diphosphoribose at the transient receptor potential melastatin 2 (TRPM2) channel: rational design of antagonists.
Chemotaxis of mouse bone marrow neutrophils and dendritic cells is controlled by adp-ribose, the major product generated by the CD38 enzyme reaction.
Chemotaxis of mouse bone marrow neutrophils and dendritic cells is controlled by adp-ribose, the major product generated by the CD38 enzyme reaction.
Gattkowski E, Johnsen A, Bauche A, Möckl F, Kulow F, Garcia Alai M, Rutherford TJ, Fliegert R, Tidow H
Biochimica et biophysica acta. Molecular cell research 2019 Jul;1866(7):1162-1170
Biochimica et biophysica acta. Molecular cell research 2019 Jul;1866(7):1162-1170
Structure-activity relationship of adenosine 5'-diphosphoribose at the transient receptor potential melastatin 2 (TRPM2) channel: rational design of antagonists.
Moreau C, Kirchberger T, Swarbrick JM, Bartlett SJ, Fliegert R, Yorgan T, Bauche A, Harneit A, Guse AH, Potter BV
Journal of medicinal chemistry 2013 Dec 27;56(24):10079-102
Journal of medicinal chemistry 2013 Dec 27;56(24):10079-102
Chemotaxis of mouse bone marrow neutrophils and dendritic cells is controlled by adp-ribose, the major product generated by the CD38 enzyme reaction.
Partida-Sanchez S, Gasser A, Fliegert R, Siebrands CC, Dammermann W, Shi G, Mousseau BJ, Sumoza-Toledo A, Bhagat H, Walseth TF, Guse AH, Lund FE
Journal of immunology (Baltimore, Md. : 1950) 2007 Dec 1;179(11):7827-39
Journal of immunology (Baltimore, Md. : 1950) 2007 Dec 1;179(11):7827-39
Chemotaxis of mouse bone marrow neutrophils and dendritic cells is controlled by adp-ribose, the major product generated by the CD38 enzyme reaction.
Partida-Sanchez S, Gasser A, Fliegert R, Siebrands CC, Dammermann W, Shi G, Mousseau BJ, Sumoza-Toledo A, Bhagat H, Walseth TF, Guse AH, Lund FE
Journal of immunology (Baltimore, Md. : 1950) 2007 Dec 1;179(11):7827-39
Journal of immunology (Baltimore, Md. : 1950) 2007 Dec 1;179(11):7827-39
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRPM2 Antibody [NB500-241] - Membrane Prep (50 ug) from Jurkat cells. Antibody: Affinity purified rabbit anti-TRPM2 antibody used for WB at 2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: TRPM2 Antibody [NB500-241] - Detection of human TRPM2 by western blot of immunoprecipitates. Samples: Membrane Prep (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-TRPM2 antibody NB500-241 used for IP at 6 ug per reaction. TRPM2 was also immunoprecipitated by rabbit anti-TRPM2 antibody NB500-242. For blotting immunoprecipitated TRPM2, NB500-241 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
