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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PA5-92294 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HAUS6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: DEPPTQNQSD LLNKKVICKQ DLECLAFTKL SETSRMETFS PAVGNRIDVM GGSEEEFMKI LDHLEVSCNK PSTNKTMLWN SFQISSGISS KSFKDNDFGI LHETLPEEVG HLSFNSSSSS EANFKLEPNS PMHGGTLLED VVGGRQTTPE SDFNLQALRS RYEALKKSLS KKREESYLSN SQTPERHKPE LSPTPQNVQT DDTLNFLDTC DLHTEHIKPS LRTSIGERKR SLSPLIKFSP VEQRLRTTIA CSLGELPNLK EEDILNKSLD AKEPPSDLTR; Positive Samples: Mouse spleen, Mouse thymus; Cellular Location: Cytoplasm, centrosome, cytoskeleton, microtubule organizing center, spindle
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.11 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using HAUS6 Polyclonal antibody (Product # PA5-92294) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 10s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of HAUS6 in extracts of various cell lines using HAUS6 Polyclonal Antibody (Product # PA5-92294) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of HAUS6 was performed in U2OS cells using HAUS6 Polyclonal Antibody (Product # PA5-92294) at a dilution of 1:100. Blue: DAPI for nuclear staining.
