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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-31257 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HAUS6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Jurkat, 293T, A431, HeLa. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.0 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HAUS6 Immunofluorescence using HAUS6 Polyclonal Antibody (Product # PA5-31257) at 1: 500; red) co-localizes at the mitotic spindle with Hice1-GFP (HAUS-GFP; green) in U2OS cells. DAPI staining (blue) shows mitotic chromosomes. Arrows indicate spindle poles.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HAUS6 Polyclonal Antibody detects HAUS6 protein on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. HAUS6 Polyclonal Antibody (Product # PA5-31257) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
