Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-96283 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RPH3AL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: KYILPLKTPG RADDPHFRPL PTEPAEREPR SSETSRIYTW ARGRVVSSDS DSDSDLSSSS LEDRLPSTGV RDRKGDKPWK ESGGSVEAPR MGFTHPPGHL SGCQSSLASG ETGTGSADPP GGPRPGLTRR APVKDTPGRA PAADAAPAGP SSCLG; Positive Samples: BT-474, 22Rv1, SW620, NCI-H460; Cellular Location: Cytoplasm, Cytoplasmic vesicle, secretory vesicle membrane
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 2.45 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RPH3AL in extracts of various cell lines. Samples were incubated with RPH3AL Polyclonal antibody (Product # PA5-96283) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of RPH3AL in L929 cells. Samples were incubated with RPH3AL Polyclonal antibody (Product # PA5-96283) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of RPH3AL in U2OS cells. Samples were incubated with RPH3AL Polyclonal antibody (Product # PA5-96283) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
