PA5-96283

antibody from Invitrogen Antibodies

Targeting: RPH3AL Noc2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96283

Provider

Invitrogen Antibodies

Product name

RPH3AL Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: KYILPLKTPG RADDPHFRPL PTEPAEREPR SSETSRIYTW ARGRVVSSDS DSDSDLSSSS LEDRLPSTGV RDRKGDKPWK ESGGSVEAPR MGFTHPPGHL SGCQSSLASG ETGTGSADPP GGPRPGLTRR APVKDTPGRA PAADAAPAGP SSCLG; Positive Samples: BT-474, 22Rv1, SW620, NCI-H460; Cellular Location: Cytoplasm, Cytoplasmic vesicle, secretory vesicle membrane

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.45 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RPH3AL in extracts of various cell lines using RPH3AL Polyclonal Antibody (Product # PA5-96283) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RPH3AL in paraffin-embedded human liver using RPH3AL Polyclonal Antibody (Product # PA5-96283) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RPH3AL in paraffin-embedded human stomach using RPH3AL Polyclonal Antibody (Product # PA5-96283) at a dilution of 1:200.