18065-1-AP

antibody from Proteintech Group

Targeting: ALDOB

Provider product page for 18065-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [42]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunoprecipitation [1]
Immunohistochemistry [2]

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Product number: 18065-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#18065-1-AP, RRID:AB_2273968
Product name: ALDOB antibody
Antibody type: Polyclonal
Description: KD/KO validated ALDOB antibody (Cat. #18065-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

ALDOB protein structure - 18065-1-AP shown in red.

Submitted references Maternal Fructose Intake During Pregnancy Induced the Hepatic Glucose Homeostasis Imbalance in the Offspring by Inhibiting Glucokinase.

Miao Y, Meng Z, Chen S, Li X, Fang T, Deng X, Sun B, Chen L
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2026 Jan 31;40(2):e71426

ALDOB is a prognostic biomarker and a potential immunotherapy target for clear cell renal cell carcinoma.

Xu W, Wu D, Li C, Yan L, Peng B, Luo Y, Liu D, Li Q, Wang T
PeerJ 2025;13:e19869

Circulating glycerate predicts resilience to fructose-induced hepatic steatosis.

Ramirez CB, Ahn IS, Rubtsova VI, Cely I, Le J, Kim J, Jung S, Kelly ME, Kim Y, Bae H, Song WS, Alam YH, Zhang G, Diamante G, Chao A, Hoffner L, Anica A, Le I, Lopez ML, Tamburini IJ, Moyer EM, Tsai A, Yang Q, Dai X, Piomelli D, Lee G, Yang X, Jang C
Cell metabolism 2025 May 6;37(5):1223-1234.e5

TRIM2 promotes metabolic adaptation to glutamine deprivation via enhancement of CPT1A activity.

Liao K, Liu K, Wang Z, Zhao K, Mei Y
The FEBS journal 2025 Jan;292(2):275-293

AMPK-regulated glycerol excretion maintains metabolic crosstalk between reductive and energetic stress.

Zhai X, Yang R, Chu Q, Guo Z, Hou P, Li X, Bai C, Lu Z, Qiao L, Fu Y, Niu J, Li B
Nature cell biology 2025 Jan;27(1):141-153

Glucose starvation mimetic aldometanib removes immune barriers permitting mice with hepatocellular carcinoma to live to normal ages.

Hu HH, Wang X, Lan B, Cheng H, Wen H, Chen F, Wu J, Li M, Chen J, Zhang J, Chen D, Lin S, Lin J, Yang M, Wu Z, Zheng ZZ, Chen F, Zhou J, Chen G, Chen Y, Deng X, Zhang CS, Liu J, Lin SC
Cell research 2025 Dec;35(12):934-953

Fructose-induced progression of steatohepatitis involves disrupting aldolase B-AMPK signaling in methionine adenosyltransferase 1A deficient mice.

Barbier-Torres L, Luque-Urbano M, Chhimwal J, Robinson AE, Fernández-Ramos D, Lopitz-Otsoa F, Van Eyk JE, Millet O, Mato JM, Lu SC
Metabolism: clinical and experimental 2025 Apr;165:156154

Multi-omics analysis of a case of congenital microtia reveals aldob and oxidative stress associated with microtia etiology.

Liu W, Wu Y, Ma R, Zhu X, Wang R, He L, Shu M
Orphanet journal of rare diseases 2024 May 27;19(1):218

MRTO4 Enhances Glycolysis to Facilitate HCC Progression by Inhibiting ALDOB.

Zheng Y, Huang Y, Li W, Cheng H
Medical science monitor : international medical journal of experimental and clinical research 2024 May 23;30:e944685

Energy stress-induced circDDX21 promotes glycolysis and facilitates hepatocellular carcinogenesis.

Luo J, Yang Y, Zhang G, Fang D, Liu K, Mei Y, Wang F
Cell death & disease 2024 May 21;15(5):354

MCT1-governed pyruvate metabolism is essential for antibody class-switch recombination through H3K27 acetylation.

Chi W, Kang N, Sheng L, Liu S, Tao L, Cao X, Liu Y, Zhu C, Zhang Y, Wu B, Chen R, Cheng L, Wang J, Sun X, Liu X, Deng H, Yang J, Li Z, Liu W, Chen L
Nature communications 2024 Jan 2;15(1):163

DUSP4 enhances therapeutic sensitivity in HER2-positive breast cancer by inhibiting the G6PD pathway and ROS metabolism by interacting with ALDOB.

Wang X, Chi W, Ma Y, Zhang Q, Xue J, Shao ZM, Xiu B, Wu J, Chi Y
Translational oncology 2024 Aug;46:102016

A pro-metastatic tRNA fragment drives aldolase A oligomerization to enhance aerobic glycolysis in lung adenocarcinoma.

Wang Q, Song X, Zhang Y, Liang S, Zhang M, Wang H, Feng Y, Li R, Ding H, Chen Y, Xia W, Dong G, Xu L, Mao Q, Jiang F
Cell reports 2024 Aug 27;43(8):114550

Aldolase A Promotes Colorectal Cancer Progression through Targeting COPS6 and Regulating MAPK Signaling Pathway.

Lu Y, Zhang Y, Wang X, Zhang H, Zhu Y, Zhang J, Sha H, Zou R, Gan Y, Sui Y, Wang J, Du T, Wu J, Feng J
Disease markers 2023;2023:1702125

ALDOB plays a tumor-suppressive role by inhibiting AKT activation in gastric cancer.

Peng C, Yang X, Li X, Ye Z, Wang J, Wu W
Journal of Cancer 2023;14(12):2255-2262

The diapause-like colorectal cancer cells induced by SMC4 attenuation are characterized by low proliferation and chemotherapy insensitivity.

Sun X, He L, Liu H, Thorne RF, Zeng T, Liu L, Zhang B, He M, Huang Y, Li M, Gao E, Ma M, Cheng C, Meng F, Lang C, Li H, Xiong W, Pan S, Ren D, Dang B, Yang Y, Wu M, Liu L
Cell metabolism 2023 Sep 5;35(9):1563-1579.e8

Metabolic classification suggests the GLUT1/ALDOB/G6PD axis as a therapeutic target in chemotherapy-resistant pancreatic cancer.

Li Y, Tang S, Shi X, Lv J, Wu X, Zhang Y, Wang H, He J, Zhu Y, Ju Y, Zhang Y, Guo S, Yang W, Yin H, Chen L, Gao D, Jin G
Cell reports. Medicine 2023 Sep 19;4(9):101162

Aldolase B-driven lactagenesis and CEACAM6 activation promote cell renewal and chemoresistance in colorectal cancer through the Warburg effect.

Chu YD, Cheng LC, Lim SN, Lai MW, Yeh CT, Lin WR
Cell death & disease 2023 Oct 10;14(10):660

RecQ dysfunction contributes to social and depressive-like behavior and affects aldolase activity in mice.

Hou Y, Park JH, Dan X, Chu X, Yang B, Hussain M, Croteau DL, Bohr VA
Neurobiology of disease 2023 May;180:106092

Aldolase B attenuates clear cell renal cell carcinoma progression by inhibiting CtBP2.

Tan M, Pan Q, Wu Q, Li J, Wang J
Frontiers of medicine 2023 Jun;17(3):503-517

Fructose-1,6-bisphosphatase is a nonenzymatic safety valve that curtails AKT activation to prevent insulin hyperresponsiveness.

Gu L, Zhu Y, Watari K, Lee M, Liu J, Perez S, Thai M, Mayfield JE, Zhang B, Cunha E Rocha K, Li F, Kim LC, Jones AC, Wierzbicki IH, Liu X, Newton AC, Kisseleva T, Lee JH, Ying W, Gonzalez DJ, Saltiel AR, Simon MC, Karin M
Cell metabolism 2023 Jun 6;35(6):1009-1021.e9

Cyclin D1 extensively reprograms metabolism to support biosynthetic pathways in hepatocytes.

Wu H, Kren BT, Lane AN, Cassel TA, Higashi RM, Fan TWM, Scaria GS, Shekels LL, Klein MA, Albrecht JH
The Journal of biological chemistry 2023 Dec;299(12):105407

Hepatic but not Intestinal FBP1 Is Required for Fructose Metabolism and Tolerance.

Bai Q, Liu Y, Wang CM, Wang JR, Feng Y, Ma X, Yang X, Shi YN, Zhang WJ
Endocrinology 2023 Apr 17;164(6)

The aldolase inhibitor aldometanib mimics glucose starvation to activate lysosomal AMPK.

Zhang CS, Li M, Wang Y, Li X, Zong Y, Long S, Zhang M, Feng JW, Wei X, Liu YH, Zhang B, Wu J, Zhang C, Lian W, Ma T, Tian X, Qu Q, Yu Y, Xiong J, Liu DT, Wu Z, Zhu M, Xie C, Wu Y, Xu Z, Yang C, Chen J, Huang G, He Q, Huang X, Zhang L, Sun X, Liu Q, Ghafoor A, Gui F, Zheng K, Wang W, Wang ZC, Yu Y, Zhao Q, Lin SY, Wang ZX, Piao HL, Deng X, Lin SC
Nature metabolism 2022 Oct;4(10):1369-1401

ATF4-dependent fructolysis fuels growth of glioblastoma multiforme.

Chen C, Zhang Z, Liu C, Wang B, Liu P, Fang S, Yang F, You Y, Li X
Nature communications 2022 Oct 16;13(1):6108

O-GlcNAc modification of leucyl-tRNA synthetase 1 integrates leucine and glucose availability to regulate mTORC1 and the metabolic fate of leucine.

Kim K, Yoo HC, Kim BG, Kim S, Sung Y, Yoon I, Yu YC, Park SJ, Kim JH, Myung K, Hwang KY, Kim S, Han JM
Nature communications 2022 May 25;13(1):2904

Uncoupling protein 2 and aldolase B impact insulin release by modulating mitochondrial function and Ca(2+) release from the ER.

Inoue R, Tsuno T, Togashi Y, Okuyama T, Sato A, Nishiyama K, Kyohara M, Li J, Fukushima S, Kin T, Miyashita D, Shiba Y, Atobe Y, Kiyonari H, Bando K, Shapiro AMJ, Funakoshi K, Kulkarni RN, Terauchi Y, Shirakawa J
iScience 2022 Jul 15;25(7):104603

Proteogenomic characterization identifies clinically relevant subgroups of intrahepatic cholangiocarcinoma.

Dong L, Lu D, Chen R, Lin Y, Zhu H, Zhang Z, Cai S, Cui P, Song G, Rao D, Yi X, Wu Y, Song N, Liu F, Zou Y, Zhang S, Zhang X, Wang X, Qiu S, Zhou J, Wang S, Zhang X, Shi Y, Figeys D, Ding L, Wang P, Zhang B, Rodriguez H, Gao Q, Gao D, Zhou H, Fan J
Cancer cell 2022 Jan 10;40(1):70-87.e15

PFKP Activation Ameliorates Foot Process Fusion in Podocytes in Diabetic Kidney Disease.

Zhang Z, Liang W, Luo Q, Hu H, Yang K, Hu J, Chen Z, Zhu J, Feng J, Zhu Z, Chi Q, Ding G
Frontiers in endocrinology 2021;12:797025

Multi-omics profiling of living human pancreatic islet donors reveals heterogeneous beta cell trajectories towards type 2 diabetes.

Wigger L, Barovic M, Brunner AD, Marzetta F, Schöniger E, Mehl F, Kipke N, Friedland D, Burdet F, Kessler C, Lesche M, Thorens B, Bonifacio E, Legido-Quigley C, Barbier Saint Hilaire P, Delerive P, Dahl A, Klose C, Gerl MJ, Simons K, Aust D, Weitz J, Distler M, Schulte AM, Mann M, Ibberson M, Solimena M
Nature metabolism 2021 Jul;3(7):1017-1031

Single-Cell Transcriptome Analysis Reveals Intratumoral Heterogeneity in ccRCC, which Results in Different Clinical Outcomes.

Hu J, Chen Z, Bao L, Zhou L, Hou Y, Liu L, Xiong M, Zhang Y, Wang B, Tao Z, Chen K
Molecular therapy : the journal of the American Society of Gene Therapy 2020 Jul 8;28(7):1658-1672

Measurement of Organ-Specific and Acute-Phase Blood Protein Levels in Early Lyme Disease.

Zhou Y, Qin S, Sun M, Tang L, Yan X, Kim TK, Caballero J, Glusman G, Brunkow ME, Soloski MJ, Rebman AW, Scavarda C, Cooper D, Omenn GS, Moritz RL, Wormser GP, Price ND, Aucott JN, Hood L
Journal of proteome research 2020 Jan 3;19(1):346-359

Loss of hepatic aldolase B activates Akt and promotes hepatocellular carcinogenesis by destabilizing the Aldob/Akt/PP2A protein complex.

He X, Li M, Yu H, Liu G, Wang N, Yin C, Tu Q, Narla G, Tao Y, Cheng S, Yin H
PLoS biology 2020 Dec;18(12):e3000803

Plasma Protein Comparison between Dairy Cows with Inactive Ovaries and Estrus.

Zhao C, Shu S, Bai Y, Wang D, Xia C, Xu C
Scientific reports 2019 Sep 23;9(1):13709

Accumulation of fructose 1,6-bisphosphate protects clear cell renal cell carcinoma from oxidative stress.

Wang J, Wu Q, Qiu J
Laboratory investigation; a journal of technical methods and pathology 2019 Jun;99(6):898-908

SIRT5-mediated SDHA desuccinylation promotes clear cell renal cell carcinoma tumorigenesis.

Ma Y, Qi Y, Wang L, Zheng Z, Zhang Y, Zheng J
Free radical biology & medicine 2019 Apr;134:458-467

The Expression of Aldolase B in Islets Is Negatively Associated With Insulin Secretion in Humans.

Gerst F, Jaghutriz BA, Staiger H, Schulte AM, Lorza-Gil E, Kaiser G, Panse M, Haug S, Heni M, Schütz M, Stadion M, Schürmann A, Marzetta F, Ibberson M, Sipos B, Fend F, Fleming T, Nawroth PP, Königsrainer A, Nadalin S, Wagner S, Peter A, Fritsche A, Richter D, Solimena M, Häring HU, Ullrich S, Wagner R
The Journal of clinical endocrinology and metabolism 2018 Dec 1;103(12):4373-4383

Proteomics and metabolomics analysis of hepatic mitochondrial metabolism in alcohol-preferring and non-preferring rats.

Zeng HL, Yang Q, Du H, Li H, Shen Y, Liu T, Chen X, Kamal GM, Guan Q, Cheng L, Wang J, Xu F
Oncotarget 2017 Nov 24;8(60):102020-102032

CUE domain-containing protein 2 promotes the Warburg effect and tumorigenesis.

Zhong X, Tian S, Zhang X, Diao X, Dong F, Yang J, Li Z, Sun L, Wang L, He X, Wu G, Hu X, Wang L, Song L, Zhang H, Pan X, Li A, Gao P
EMBO reports 2017 May;18(5):809-825

Fructose-1,6-bisphosphate and aldolase mediate glucose sensing by AMPK.

Zhang CS, Hawley SA, Zong Y, Li M, Wang Z, Gray A, Ma T, Cui J, Feng JW, Zhu M, Wu YQ, Li TY, Ye Z, Lin SY, Yin H, Piao HL, Hardie DG, Lin SC
Nature 2017 Aug 3;548(7665):112-116

Aldolase B inhibits metastasis through Ten-Eleven Translocation 1 and serves as a prognostic biomarker in hepatocellular carcinoma.

Tao QF, Yuan SX, Yang F, Yang S, Yang Y, Yuan JH, Wang ZG, Xu QG, Lin KY, Cai J, Yu J, Huang WL, Teng XL, Zhou CC, Wang F, Sun SH, Zhou WP
Molecular cancer 2015 Sep 17;14:170

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Sun L, Song L, Wan Q, Wu G, Li X, Wang Y, Wang J, Liu Z, Zhong X, He X, Shen S, Pan X, Li A, Wang Y, Gao P, Tang H, Zhang H
Cell research 2015 Apr;25(4):429-44

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18065-1-AP(ALDOB antibody) at dilution of 1:4000
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using ALDOB antibody 18065-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP Result of anti-ALDOB (IP:18065-1-AP, 4ug; Detection:18065-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human liver using 18065-1-AP(ALDOB antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human liver using 18065-1-AP(ALDOB antibody) at dilution of 1:100 (under 40x lens)
Sample type: tissue