PA5-34659

antibody from Invitrogen Antibodies

Targeting: ALDOB

Antibody data

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Validation data

Product number: PA5-34659 - Provider product page
Provider: Invitrogen Antibodies
Product name: Aldolase B Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: 293T, A431, HeLa, HepG2, mouse brain, rat brain, Neuro2A, GL261, C8D30, PC-12. Predicted reactivity: Mouse (96%), Rat (97%), Rabbit (98%), Chicken (84%), Sheep (90%), Bovine (91%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.09 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

ALDOB protein structure - PA5-34659 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Aldolase B using 30 µg of A) 293T (B) A431 and C) HeLa lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an Aldolase B polyclonal antibody (Product # PA5-34659) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Aldolase B was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Aldolase B Polyclonal Antibody (Product # PA5-34659) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Aldolase B was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Aldolase B Polyclonal Antibody (Product # PA5-34659) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Aldolase B was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Aldolase B Polyclonal Antibody (Product # PA5-34659) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Aldolase B was performed by separating 50 µg of various tissue extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Aldolase B Polyclonal Antibody (Product # PA5-34659) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Aldolase B Polyclonal Antibody detects ALDOB protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract. B. 30 µg GL261 whole cell lysate/extract. C. 30 µg C8D30 whole cell lysate/extract.10% SDS-PAGE. Aldolase B Polyclonal Antibody (Product # PA5-34659) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Aldolase B Polyclonal Antibody detects ALDOB protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract.10% SDS-PAGE. Aldolase B Polyclonal Antibody (Product # PA5-34659) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.