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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-30218 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Aldolase B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, Jurkat, Raji, mouse liver, PC-12, Rat2, mouse brain, rat brain.
- Concentration
- 0.59 mg/mL
Submitted references Aldolase B-Mediated Fructose Metabolism Drives Metabolic Reprogramming of Colon Cancer Liver Metastasis.
Bu P, Chen KY, Xiang K, Johnson C, Crown SB, Rakhilin N, Ai Y, Wang L, Xi R, Astapova I, Han Y, Li J, Barth BB, Lu M, Gao Z, Mines R, Zhang L, Herman M, Hsu D, Zhang GF, Shen X
Cell metabolism 2018 Jun 5;27(6):1249-1262.e4
Cell metabolism 2018 Jun 5;27(6):1249-1262.e4
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Aldolase B was performed by separating 50 µg of various tissue extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Aldolase B Polyclonal Antibody (Product # PA5-30218) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Aldolase B Polyclonal Antibody (Product # PA5-30218). Sample (30 µg of whole cell lysate). Lane A: 293T. Lane B: A431. Lane C: JurKat. Lane D: Raji. 10% SDS PAGE. Aldolase B Polyclonal Antibody (Product # PA5-30218) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Aldolase B Polyclonal Antibody (Product # PA5-30218). Sample (20 µg of whole cell lysate). Lane A: mouse liver. 10% SDS PAGE. Aldolase B Polyclonal Antibody (Product # PA5-30218) diluted at 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Aldolase B Polyclonal Antibody detects ALDOB protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract. B. 30 µg Rat2 whole cell lysate/extract.10 % SDS-PAGE. Aldolase B Polyclonal Antibody (Product # PA5-30218) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Aldolase B in methanol-fixed A431 cells using an Aldolase B polyclonal antibody (Product # PA5-30218) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Aldolase B Polyclonal Antibody detects ALDOB protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma tissue. Aldolase B Polyclonal Antibody (Product # PA5-30218) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
