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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-55079 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- VPS53 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: ETFQKILDMK GLKRSEQSSM LELLRQRLPA PPSGAESSGS LSLTAPTPEQ ESSRIRKLEK LIK Highest antigen sequence identity to the following orthologs: Mouse - 92%, Rat - 94%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of VPS53 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a VPS53 Polyclonal Antibody (Product # PA5-55079).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of VPS53 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a VPS53 Polyclonal Antibody (Product # PA5-55079).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of VPS53 in human cell line U-2 OS using a VPS53 Polyclonal Antibody (Product # PA5-55079) shows localization to cytosol and the Golgi apparatus.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of VPS53 in human duodenum using a VPS53 Polyclonal Antibody (Product # PA5-55079) shows strong cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of VPS53 in human cerebral cortex using VPS53 Polyclonal Antibody (Product # PA5-55079) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of VPS53 in human pancreas using VPS53 Polyclonal Antibody (Product # PA5-55079) shows low expression as expected.
