PA5-55079

antibody from Invitrogen Antibodies

Targeting: VPS53 FLJ10979, HCCS1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-55079

Provider

Invitrogen Antibodies

Product name

VPS53 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: ETFQKILDMK GLKRSEQSSM LELLRQRLPA PPSGAESSGS LSLTAPTPEQ ESSRIRKLEK LIK

Concentration

0.2 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of VPS53 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a VPS53 Polyclonal Antibody (Product # PA5-55079).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of VPS53 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a VPS53 Polyclonal Antibody (Product # PA5-55079).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of VPS53 in human cell line U-2 OS using a VPS53 Polyclonal Antibody (Product # PA5-55079) shows localization to cytosol and the Golgi apparatus.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of VPS53 in human duodenum using a VPS53 Polyclonal Antibody (Product # PA5-55079) shows strong cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of VPS53 in human cerebral cortex using VPS53 Polyclonal Antibody (Product # PA5-55079) shows high expression.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of VPS53 in human pancreas using VPS53 Polyclonal Antibody (Product # PA5-55079) shows low expression as expected.