F54974

antibody from NSJ Bioreagents

Targeting: VPS53 FLJ10979, HCCS1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54974

Provider

NSJ Bioreagents

Product name

VPS53 Antibody

Antibody type

Polyclonal

Description

This highly specific VPS53 antibody is suitable for use in Flow cytometry/Immunohistochemistry/Western blot applications with human and mouse samples.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the VPS53 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of mouse brain tissue lysate with VPS53 antibody. Predicted molecular weight ~94 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human MCF7 cell lysate with VPS53 antibody. Predicted molecular weight ~94 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human brain tissue with VPS53 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human MDA-MB-468 cells with VPS53 antibody; Blue=isotype control, Green= VPS53 antibody.