Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- TA322140 - Provider product page
- Provider
- OriGene
- Product name
- Anti-KISS1R Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to a region derived from 383-398 amino acids of Human KISS1 receptor
- Description
- Anti-KISS1R Rabbit Polyclonal Antibody
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.5mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Predicted band size: 43 kDa . Positive control: Jurkat, Hela, 231 cell and Mouse pancreas tissue lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lane1: Jurkat cell lysate Lane2: Hela cell lysate Lane3: 231 cell lysate Lane4: Mouse pancreas tissue lysate Lysates: 40 ug per lane Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 minutes)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Predicted cell location: Cytoplasm, Cell membrane. Positive control: Human ovarian cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using KISS1R antibody at dilution 1/15, on the right is treated with the synthetic peptide. (Original magnification:x200)
- Validation comment
- IHC