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LearnPA5-35187
antibody from Invitrogen Antibodies
Targeting: RBPJ
CBF1, IGKJRB, IGKJRB1, KBF2, RBP-J, RBPJK, RBPSUH, SUH
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-35187 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RBPJ Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Multiple Immunostainings with Different Epitope Retrievals-The FOLGAS Protocol.
von Schoenfeld A, Bronsert P, Poc M, Fuller A, Filby A, Kraft S, Kurowski K, Sörensen K, Huber J, Pfeiffer J, Proietti M, Stehl V, Werner M, Seidl M
International journal of molecular sciences 2021 Dec 25;23(1)
International journal of molecular sciences 2021 Dec 25;23(1)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RBPJ in Ramos cell lysate (35 µg/lane) using a RBPJ polyclonal antibody (Product # PA5-35187).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of RBPJ showing staining in the nucleus of A2058 cells using a RBPJ polyclonal antibody (Product # PA5-35187) followed by detection using a fluorescent conjugated secondary antibody (green). Cytoplasmic actin was stained with a fluorescent red phalloidin (7units/mL, 1 h at 37°C). Nuclei were stained with DAPI (blue) (10 µg/mL, 10 min).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of RBPJ in formalin-fixed, paraffin-embedded human stomach tissue using a RBPJ polyclonal antibody (Product # PA5-35187) followed by peroxidase conjugation of the secondary antibody and DAB staining.
