695402

antibody from BioLegend

Targeting: RBPJ CBF1, IGKJRB, IGKJRB1, KBF2, RBP-J, RBPJK, RBPSUH, SUH

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

695402

Provider

BioLegend

Proper citation

BioLegend Cat#695402, RRID:AB_2650803

Product name

Purified anti-RBPJ

Antibody type

Monoclonal

Reactivity

Human

Host

Rat

Conjugate

Unconjugated

Antibody clone number

W16038A

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Total cell lysates (15µg protein) from MCF-7, HeLa, NIH3T3 were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1µg/mL purified anti-RBPJ antibody (clone W16038A) antibody (upper). Proteins were visualized using a goat anti-rat-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower). M*: protein standards with longer exposure time.

Conjugate

Unconjugated