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LearnPA5-22226
antibody from Invitrogen Antibodies
Targeting: RBPJ
CBF1, IGKJRB, IGKJRB1, KBF2, RBP-J, RBPJK, RBPSUH, SUH
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
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- Product number
- PA5-22226 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RBPJ Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, Molt-4, Raji. Predicted reactivity: Mouse (97%), Rat (98%), Xenopus laevis (93%), Rhesus Monkey (99%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.75 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RBP-Jkappa using 30 µg of Raji lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a RBP-Jkappa polyclonal antibody (Product # PA5-22226) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RBPJ was performed by separating 30 µg of Jurkat lysates by 7.5% SDS PAGE. Proteins were transferred to a membrane and probed with a RBPJ Polyclonal Antibody (Product # PA5-22226) at a dilution of 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RBPJ Polyclonal Antibody (Product # PA5-22226). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with RBPJ Polyclonal Antibody (Product # PA5-22226) diluted at 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody,.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of RBP-Jkappa in paraformaldehyde-fixed HeLa cells using a RBP-Jkappa polyclonal antibody (Product # PA5-22226) at a 1:100 dilution.
