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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [8]
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- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF-292-NA - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IGF-II/IGF2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human IGF-II/IGF2 in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant mouse IGF-II/IGF2 is observed and less than 1% cross-reactivity with recombinant human IGF-I/IGF1 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 250 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies.
A journey through growth plates: tracking differences in morphology and regulation between the spine and the long bones in a pig model.
Cancer cell-secreted IGF2 instigates fibroblasts and bone marrow-derived vascular progenitor cells to promote cancer progression.
A subset of bone marrow stromal cells regulate ATP-binding cassette gene expression via insulin-like growth factor-I in a leukemia cell line.
'Big'-insulin-like growth factor-II signaling is an autocrine survival pathway in gastrointestinal stromal tumors.
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Insulin-like growth factor-II is increased in systemic sclerosis-associated pulmonary fibrosis and contributes to the fibrotic process via Jun N-terminal kinase- and phosphatidylinositol-3 kinase-dependent pathways.
The role of matrix metalloproteinase-7 in redefining the gastric microenvironment in response to Helicobacter pylori.
Jalili R, Horecka J, Swartz JR, Davis RW, Persson HHJ
Proceedings of the National Academy of Sciences of the United States of America 2018 Jan 30;115(5):E925-E933
Proceedings of the National Academy of Sciences of the United States of America 2018 Jan 30;115(5):E925-E933
A journey through growth plates: tracking differences in morphology and regulation between the spine and the long bones in a pig model.
Raimann A, Javanmardi A, Egerbacher M, Haeusler G
The spine journal : official journal of the North American Spine Society 2017 Nov;17(11):1674-1684
The spine journal : official journal of the North American Spine Society 2017 Nov;17(11):1674-1684
Cancer cell-secreted IGF2 instigates fibroblasts and bone marrow-derived vascular progenitor cells to promote cancer progression.
Xu WW, Li B, Guan XY, Chung SK, Wang Y, Yip YL, Law SY, Chan KT, Lee NP, Chan KW, Xu LY, Li EM, Tsao SW, He QY, Cheung AL
Nature communications 2017 Feb 10;8:14399
Nature communications 2017 Feb 10;8:14399
A subset of bone marrow stromal cells regulate ATP-binding cassette gene expression via insulin-like growth factor-I in a leukemia cell line.
Benabbou N, Mirshahi P, Bordu C, Faussat AM, Tang R, Therwath A, Soria J, Marie JP, Mirshahi M
International journal of oncology 2014 Oct;45(4):1372-80
International journal of oncology 2014 Oct;45(4):1372-80
'Big'-insulin-like growth factor-II signaling is an autocrine survival pathway in gastrointestinal stromal tumors.
Rikhof B, van der Graaf WT, Suurmeijer AJ, van Doorn J, Meersma GJ, Groenen PJ, Schuuring EM, Meijer C, de Jong S
The American journal of pathology 2012 Jul;181(1):303-12
The American journal of pathology 2012 Jul;181(1):303-12
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Gonzalez RM, Seurynck-Servoss SL, Crowley SA, Brown M, Omenn GS, Hayes DF, Zangar RC
Journal of proteome research 2008 Jun;7(6):2406-14
Journal of proteome research 2008 Jun;7(6):2406-14
Insulin-like growth factor-II is increased in systemic sclerosis-associated pulmonary fibrosis and contributes to the fibrotic process via Jun N-terminal kinase- and phosphatidylinositol-3 kinase-dependent pathways.
Hsu E, Feghali-Bostwick CA
The American journal of pathology 2008 Jun;172(6):1580-90
The American journal of pathology 2008 Jun;172(6):1580-90
The role of matrix metalloproteinase-7 in redefining the gastric microenvironment in response to Helicobacter pylori.
McCaig C, Duval C, Hemers E, Steele I, Pritchard DM, Przemeck S, Dimaline R, Ahmed S, Bodger K, Kerrigan DD, Wang TC, Dockray GJ, Varro A
Gastroenterology 2006 May;130(6):1754-63
Gastroenterology 2006 May;130(6):1754-63
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- IGF-II/IGF2 in Human Placenta. IGF-II/IGF2 was detected in immersion fixed paraffin-embedded sections of human placenta (chorionic villi) using 5 µg/mL Goat Anti-Human IGF-II/IGF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-292-NA) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IGF-II/IGF2 and Neutralization by Human IGF-II/IGF2 Antibody. Recombinant Human IGF-II/IGF2 (Catalog # 292-G2) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IGF-II/IGF2 (14 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IGF-II/IGF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-292-NA). The ND50 is typically 0.2-0.8 µg/mL.
