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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB292-100 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal IGF-II/IGF2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human IGF-II/IGF2 in direct ELISA and Western blots. This antibody shows 100% cross-reactivity with recombinant mouse (rm) IGF-II and no cross-reactivity with recombinant human IGF-I or rmIGF-I.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Cell Proliferation Induced by IGF-II/IGF2 and Neutralization by Human IGF-II/IGF2 Antibody. Recombinant Human IGF-II/IGF2 (Catalog # 292-G2) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IGF-II/IGF2 (14 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IGF-II/IGF2 Monoclonal Antibody (Catalog # MAB292). The ND50 is typically 2-12 µg/mL.
