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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB292-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IGF-II/IGF2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human IGF-II/IGF2 in direct ELISA and Western blots. This antibody shows 100% cross-reactivity with recombinant mouse (rm) IGF-II and no cross-reactivity with recombinant human IGF-I or rmIGF-I.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P01344.1- Isotype
- IgG
- Antibody clone number
- 75015
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Immune targeting of autocrine IGF2 hampers rhabdomyosarcoma growth and metastasis.
Matrix metalloproteinase-1 facilitates MSC migration via cleavage of IGF-2/IGFBP2 complex.
Co-targeting the IGF system and HIF-1 inhibits migration and invasion by (triple-negative) breast cancer cells.
Association between paternally inherited haplotypes upstream of the insulin gene and umbilical cord IGF-II levels.
De Giovanni C, Nanni P, Landuzzi L, Ianzano ML, Nicoletti G, Croci S, Palladini A, Lollini PL
BMC cancer 2019 Feb 7;19(1):126
BMC cancer 2019 Feb 7;19(1):126
Matrix metalloproteinase-1 facilitates MSC migration via cleavage of IGF-2/IGFBP2 complex.
Guan SP, Lam ATL, Newman JP, Chua KLM, Kok CYL, Chong ST, Chua MLK, Lam PYP
FEBS open bio 2018 Jan;8(1):15-26
FEBS open bio 2018 Jan;8(1):15-26
Co-targeting the IGF system and HIF-1 inhibits migration and invasion by (triple-negative) breast cancer cells.
Mancini M, Gariboldi MB, Taiana E, Bonzi MC, Craparotta I, Pagin M, Monti E
British journal of cancer 2014 Jun 10;110(12):2865-73
British journal of cancer 2014 Jun 10;110(12):2865-73
Association between paternally inherited haplotypes upstream of the insulin gene and umbilical cord IGF-II levels.
Adkins RM, Fain JN, Krushkal J, Klauser CK, Magann EF, Morrison JC
Pediatric research 2007 Oct;62(4):451-5
Pediatric research 2007 Oct;62(4):451-5
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by IGF-II/IGF2 and Neutralization by Human IGF-II/IGF2 Antibody. Recombinant Human IGF-II/IGF2 (Catalog # 292-G2) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IGF-II/IGF2 (14 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IGF-II/IGF2 Monoclonal Antibody (Catalog # MAB292). The ND50 is typically 2-12 µg/mL.
