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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MA5-12252 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Pds1 Monoclonal Antibody (DCS-280)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- IHC staining of formalin-fixed tissues requires boiling in 10mM citrate buffer, pH 6.0, for 10-20 minutes, followed by cooling at room temperature for 20 minutes. HeLa or tonsil tissues can be used as positive controls.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- DCS-280
- Vial size
- 500 µL
- Concentration
- 0.2 mg/mL
- Storage
- 4° C
Submitted references SUMOylation inhibits FOXM1 activity and delays mitotic transition.
Down-regulation of the PTTG1 proto-oncogene contributes to the melanoma suppressive effects of the cyclin-dependent kinase inhibitor PHA-848125.
Heat shock protein inhibitors, 17-DMAG and KNK437, enhance arsenic trioxide-induced mitotic apoptosis.
Modified cell cycle status in a mouse model of altered neuronal vulnerability (slow Wallerian degeneration; Wlds).
Mitotic arrest-associated apoptosis induced by sodium arsenite in A375 melanoma cells is BUBR1-dependent.
Securin induces genetic instability in colorectal cancer by inhibiting double-stranded DNA repair activity.
Securin and separase phosphorylation act redundantly to maintain sister chromatid cohesion in mammalian cells.
DNA damage-induced mitotic catastrophe is mediated by the Chk1-dependent mitotic exit DNA damage checkpoint.
Complete loss of the tumor suppressor MAD2 causes premature cyclin B degradation and mitotic failure in human somatic cells.
Rb inactivation promotes genomic instability by uncoupling cell cycle progression from mitotic control.
Myatt SS, Kongsema M, Man CW, Kelly DJ, Gomes AR, Khongkow P, Karunarathna U, Zona S, Langer JK, Dunsby CW, Coombes RC, French PM, Brosens JJ, Lam EW
Oncogene 2014 Aug 21;33(34):4316-29
Oncogene 2014 Aug 21;33(34):4316-29
Down-regulation of the PTTG1 proto-oncogene contributes to the melanoma suppressive effects of the cyclin-dependent kinase inhibitor PHA-848125.
Caporali S, Alvino E, Levati L, Esposito AI, Ciomei M, Brasca MG, Del Bufalo D, Desideri M, Bonmassar E, Pfeffer U, D'Atri S
Biochemical pharmacology 2012 Sep 1;84(5):598-611
Biochemical pharmacology 2012 Sep 1;84(5):598-611
Heat shock protein inhibitors, 17-DMAG and KNK437, enhance arsenic trioxide-induced mitotic apoptosis.
Wu YC, Yen WY, Lee TC, Yih LH
Toxicology and applied pharmacology 2009 Apr 15;236(2):231-8
Toxicology and applied pharmacology 2009 Apr 15;236(2):231-8
Modified cell cycle status in a mouse model of altered neuronal vulnerability (slow Wallerian degeneration; Wlds).
Wishart TM, Pemberton HN, James SR, McCabe CJ, Gillingwater TH
Genome biology 2008;9(6):R101
Genome biology 2008;9(6):R101
Mitotic arrest-associated apoptosis induced by sodium arsenite in A375 melanoma cells is BUBR1-dependent.
McNeely SC, Taylor BF, States JC
Toxicology and applied pharmacology 2008 Aug 15;231(1):61-7
Toxicology and applied pharmacology 2008 Aug 15;231(1):61-7
Securin induces genetic instability in colorectal cancer by inhibiting double-stranded DNA repair activity.
Kim DS, Franklyn JA, Smith VE, Stratford AL, Pemberton HN, Warfield A, Watkinson JC, Ishmail T, Wakelam MJ, McCabe CJ
Carcinogenesis 2007 Mar;28(3):749-59
Carcinogenesis 2007 Mar;28(3):749-59
Securin and separase phosphorylation act redundantly to maintain sister chromatid cohesion in mammalian cells.
Huang X, Hatcher R, York JP, Zhang P
Molecular biology of the cell 2005 Oct;16(10):4725-32
Molecular biology of the cell 2005 Oct;16(10):4725-32
DNA damage-induced mitotic catastrophe is mediated by the Chk1-dependent mitotic exit DNA damage checkpoint.
Huang X, Tran T, Zhang L, Hatcher R, Zhang P
Proceedings of the National Academy of Sciences of the United States of America 2005 Jan 25;102(4):1065-70
Proceedings of the National Academy of Sciences of the United States of America 2005 Jan 25;102(4):1065-70
Complete loss of the tumor suppressor MAD2 causes premature cyclin B degradation and mitotic failure in human somatic cells.
Michel L, Diaz-Rodriguez E, Narayan G, Hernando E, Murty VV, Benezra R
Proceedings of the National Academy of Sciences of the United States of America 2004 Mar 30;101(13):4459-64
Proceedings of the National Academy of Sciences of the United States of America 2004 Mar 30;101(13):4459-64
Rb inactivation promotes genomic instability by uncoupling cell cycle progression from mitotic control.
Hernando E, Nahlé Z, Juan G, Diaz-Rodriguez E, Alaminos M, Hemann M, Michel L, Mittal V, Gerald W, Benezra R, Lowe SW, Cordon-Cardo C
Nature 2004 Aug 12;430(7001):797-802
Nature 2004 Aug 12;430(7001):797-802
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of C2C12 (Lane 1), SK-N-AS (Lane 2) and Caco-2 (Lane 3).The blots were probed with Mouse Anti-IGF2 Monoclonal Antibody (Product # MA5-12252, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 26 kDa band corresponding to IGF2 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human tonsil stained with Pds1 antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of lympocytes in germinal center.
