Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AP52163PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP52163PU-N, RRID:AB_11144745
- Product name
- anti Insulin-like growth factor II / IGF2 (Center)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 46-77 amino acids from the Central region of Human IGF2.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of IGF2 Antibody Cat.-No AP52163PU-N in Hela and cell line lysates and in Mouse cerebellum tissue lysates (35µg/lane). This demonstrates the IGF2 antibody detected the IGF2 protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin fixed and paraffin embedded human placenta tissue stained with IGF2 Antibody Cat.-No AP52163PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Hela cells using IGF2 Antibody Cat.-No AP52163PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.