Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-B413 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ RAD52 Antibody LS-B413
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
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Supportive validation
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- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-Human RAD52 Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-Human RAD52 antibody to detect Human RAD52 present in a HeLa nuclear extract (panel B). ~30 ug of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a band of ~63.0 kD is detected. Peptide competition (panel A) blocks the specific staining of this band. The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] ( diluted 1:5000 for 45 min. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by.