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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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- Product number
- MA1-23334 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RAD52 Monoclonal Antibody (5H9)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: HeLa whole cell lysate.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5H9
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Rad52 in whole cell extracts (30 µg). Samples was separated by 10% SDS-PAGE and the membrane was probed with Rad52 Monoclonal antibody (Product # MA1-23334) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RAD52 in HeLa whole cell lysate using a RAD52 monoclonal antibody (Product # MA1-23334).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RAD52 Monoclonal Antibody (5H9) detects Rad52 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with RAD52 Monoclonal Antibody (5H9) (Product # MA1-23334) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
