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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- TA319514 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal anti-RAD52 antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal anti-RAD52 antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- RAD52
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
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Supportive validation
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- OriGene (provider)
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- Experimental details
- WB analysis using Anti-Human RAD52 antibody to detect Human RAD52 present in a HeLa nuclear extract (panel B).?? ~30ug of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Peptide competition (panel A) blocks the specific staining of this band.? The blot was incubated with a 1:1000 dilution of the antibody followed by detection using IRDye?800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000.
- Validation comment
- WB
