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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- PAB10230 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10230, RRID:AB_1712464
- Product name
- RAD52 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of RAD52.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Expression of human RAD52 confers resistance to ionizing radiation in mammalian cells.
The human and mouse homologs of the yeast RAD52 gene: cDNA cloning, sequence analysis, assignment to human chromosome 12p12.2-p13, and mRNA expression in mouse tissues.
Cloning of human and mouse genes homologous to RAD52, a yeast gene involved in DNA repair and recombination.
Park MS
The Journal of biological chemistry 1995 Jun 30;270(26):15467-70
The Journal of biological chemistry 1995 Jun 30;270(26):15467-70
The human and mouse homologs of the yeast RAD52 gene: cDNA cloning, sequence analysis, assignment to human chromosome 12p12.2-p13, and mRNA expression in mouse tissues.
Shen Z, Denison K, Lobb R, Gatewood JM, Chen DJ
Genomics 1995 Jan 1;25(1):199-206
Genomics 1995 Jan 1;25(1):199-206
Cloning of human and mouse genes homologous to RAD52, a yeast gene involved in DNA repair and recombination.
Muris DF, Bezzubova O, Buerstedde JM, Vreeken K, Balajee AS, Osgood CJ, Troelstra C, Hoeijmakers JH, Ostermann K, Schmidt H
Mutation research 1994 Nov;315(3):295-305
Mutation research 1994 Nov;315(3):295-305
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- Experimental details
- Western blot analysis is shown using RAD52 polyclonal antibody (Cat # PAB10230) to detect human RAD52 present in a HeLa nuclear extract (panel B).Approximately 30 ug of lysate was loaded per lane for 4-20% gradient SDS-PAGE.Comparison to a molecular weight marker (not shown) indicates a band of ~ 63.0 KDa is detected.Peptide competition (panel A) blocks the specific staining of this band.The blot was incubated with a 1 : 1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDye™800 labeled Goat-a-Rabbit IgG [H&L] diluted 1 : 5,000 for 45 min.
