Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Other assay [2]
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Validation data
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- Product number
- PA5-65036 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RAD52 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: ILGGRDSHPAA GGGSVLCFGQ CQYTAEEYQA IQKALRQRLG PEYISSRMAG GGQKVCYIEG HRVINLANEM FGYNGWAHSI TQQNVDFVDL NNGKFYVGVC AFVRVQLKDG SYHEDVGYGV SE Highest antigen sequence identity to the following orthologs - mouse 90%, rat 90%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.08 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references XAB2 promotes Ku eviction from single-ended DNA double-strand breaks independently of the ATM kinase.
Sharma AB, Erasimus H, Pinto L, Caron MC, Gopaul D, Peterlini T, Neumann K, Nazarov PV, Fritah S, Klink B, Herold-Mende CC, Niclou SP, Pasero P, Calsou P, Masson JY, Britton S, Van Dyck E
Nucleic acids research 2021 Sep 27;49(17):9906-9925
Nucleic acids research 2021 Sep 27;49(17):9906-9925
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of RAD52 in human cell line U-2 OS shows localization to nuclear speckles. Samples were probed using a RAD52 Polyclonal Antibody (Product # PA5-65036).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5. Rescue of defective XAB2 by overexpression of RAD51 and RAD52. ( A ) Immunoblotting analysis of RAD52 (upper panel) and RAD51 (lower panel) overexpression in control and XAB2-depleted U87 cells. ( B and C ) Representative micrographs of gammaH2AX foci (red) detected by immunofluorescence in the indicated cells exposed to 15 muM TMZ (or DMSO) for 2 h and allowed to recover in drug-free medium for the indicated times ( B ) and related quantification ( C ) (scale bar: 5 mum). ( D ) Percentage of Cyclin A-positive cells displaying pS1778-53BP1 foci among control and XAB2-depleted cells harboring the indicated constructs, following exposure to 15 muM TMZ (or DMSO) for 2 h and recovery in drug-free medium for the indicated times. Data are the average of n = 2 or more biological replicates (30-50 cells/sample/experiment). The images are representative of three independent biological repeats. Bars represent mean +- s.e.m. Significant differences between specified comparisons were assessed by two-ways ANOVA and are highlighted by stars (* P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001) (ns = not significant).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6. XAB2 depletion shows synthetic lethality with loss or inhibition of RAD52. ( A ) Immunoblots illustrating the efficiency of RAD52 depletion achieved by 2 independent shRNAs (shRAD52-1 and shRAD52-2) as compared to shCTRL. Actin was used as a loading control. ( B and C ) Clonogenic survival assays carried out with control and RAD52-depleted cells exposed to 15 muM TMZ (or DMSO) for 2 h ( B ) and related quantification ( C ). Cell viabilities are expressed as % relative to that of untreated cells (set at 100%). ( D ) Micrographs of control or XAB2-depleted NCH644 cells transduced with either shRAD52 or control (shCTL) shRNAs, taken at day 18 following transduction and selection in puromycin/G418 (scale bars: 250 mum). Control and XAB2-depleted cells, obtained using shRNA constructs expressing the puromycin-resistance marker, were transduced with pLKO-based constructs expressing shCTL or shRAD52-2 shRNAs, followed by additional selection with G418. No spheroid growth was observed in the double knockdown cells. ( E and F ) Quantification of the clonogenic survival of U87 cells following single or double knockdown of XAB2 and RAD52 ( E ) and representative illlustration of the plating efficiency of the double knockdown cells compared to control cells ( F ). See Supplementary Figures S1E and S5B for illustrations of the single knockdowns. ( G ) xCELLigence real-time cell proliferation analysis with control and XAB2-depleted cells exposed to the indicated concentrations of