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- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- MA5-31888 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RAD52 Monoclonal Antibody (5E11E7)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-31888 has been tested in indirect ELISA.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5E11E7
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references KMT2C-deficient tumors have elevated APOBEC mutagenesis and genomic instability in multiple cancers.
Hu X, Biswas A, De S
NAR cancer 2022 Sep;4(3):zcac023
NAR cancer 2022 Sep;4(3):zcac023
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RAD52 in HepG2 (1), MCF-7 (2), MCF-7 (3), and C6 (4) cell lysate. Samples were incubated with RAD52 monoclonal antibody (Product # MA5-31888) using a dilution of 1:500-1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RAD52 in human RAD52 recombinant protein. Sample was incubated with RAD52 monoclonal antibody (Product # MA5-31888) using a dilution of 1:500-1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RAD52 in HEK293 (1) and RAD52 cell lysate. Samples were incubated with RAD52 monoclonal antibody (Product # MA5-31888) using a dilution of 1:500-1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELISA analysis of RAD52 in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). Samples were incubated with RAD52 monoclonal antibody (Product # MA5-31888) using a dilution of 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of RAD52 in HeLa cells (green). Sample was incubated with RAD52 monoclonal antibody (Product # MA5-31888) using a dilution of 1:200-1:1000 followed by DRAQ5 fluorescent DNA dye (blue), and Alexa Fluor- 555 phalloidin (red labeled actin filaments) .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of RAD52 in MCF7 cells (green). Sample was incubated with RAD52 monoclonal antibody (Product # MA5-31888) using a dilution of 1:200-1:400 followed by negative control (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5. KMT2C mutant tumors have elevated genomic rearrangements and signatures of HR defects. ( A , B ) Immunofluorescence analysis and representative images show 53BP1 and RAD52 foci upon knockdown of indicated genes in HEK293T and RPE1 cells with error bars representing SEM. P -value was calculated using t -test; * P -value < 0.05, ** P -value < 0.01. Scale bars indicate 20 mum. DAPI was used as a nuclear counterstain. ( C ) Barplots showing the burden of somatic mutations attributed to SBS3 in KMT2C mutant and other samples in the breast cancer cohorts BRCA-EU and BRCA-FR. Plots for other cohorts are shown in Supplementary Figure S4C . P -value was calculated by Mann-Whitney U -test, * P -value < 0.05, ** P -value < 0.01. ( D ) Barplot showing the frequencies of small deletions (
