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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- MA1-91034 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Calreticulin Monoclonal Antibody (FMC 75)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Recommended positive controls: HeLa heat shocked cell lysate.
- Reactivity
- Human, Hamster
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- FMC 75
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Calreticulin using Calreticulin Monoclonal Antibody (FMC 75) (Product # MA1-91034): Lane 1: HeLa (Heat Shocked). Lane 2: Vero.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extract (30 µg lysate) of RAW 264.7 (Lane 1), SHSY5Y (Lane 2), HeLa (Lane 3), A431 (Lane 4), SKOV3 (Lane 5), IM9 (Lane 6), Jurkat (Lane 7), K562 (Lane 8) and MCF7 (Lane 9). The blot was probed with Anti-Calreticulin Monoclonal Antibody (FMC 75) (Product # MA1-91034, 1:3000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 55 kDa band corresponding to Calreticulin was observed in all human cell lines but no mouse reactivity was observed with RAW 264.7 cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of human uterus tissue stained with Calreticulin Monoclonal Antibody (FMC 75) (Product # MA1-91034) at 10 µg/mL.
