Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA336776 - Provider product page
- Provider
- OriGene
- Product name
- Mouse Monoclonal Calreticulin Antibody (1G6A7)
- Antibody type
- Monoclonal
- Description
- Mouse Monoclonal Calreticulin Antibody (1G6A7)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CALR
- Isotype
- IgG
- Antibody clone number
- 1G6A7
- Vial size
- 100 µl
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot: Calreticulin Antibody (1G6A7) [TA336776] - Western blot analysis using anti-Calreticulin mAb against Hela (1), A549 (2), NTERA2 (3) and MCF-7 (4) cell lysate.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot: Calreticulin Antibody (1G6A7) [TA336776] - Western blot analysis using Calreticulin mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Calreticulin cDNA (2).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Calreticulin Antibody (1G6A7) [TA336776] - Confocal immunofluorescence analysis of 3T3-L1 cells using anti-Calreticulin mAb (green). Blue: DRAQ5 fluorescent DNA dye.
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Calreticulin Antibody (1G6A7) [TA336776] - Confocal immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using anti-Calreticulin mAb (green). Red: Actin filaments have been labeled with DY-554 phalloi
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Calreticulin Antibody (1G6A7) [TA336776] - The Calreticulin antibody was tested in HeLa cells at a 1:250 dilution against Dylight 488 (Green). Actin was counterstained against Phalloidin 568 (Red) and cells were mou
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Calreticulin Antibody (1G6A7) [TA336776] - Immunohistochemical analysis of paraffin-embedded human placenta tissues using anti-Calreticulin mAb.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow Cytometry: Calreticulin Antibody (1G6A7) [TA336776] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Calreticulin antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6
- Validation comment
- FC