H00000811-M01

antibody from Abnova Corporation

Targeting: CALR cC1qR, CRT, FLJ26680, RO, SSA

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000811-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000811-M01, RRID:AB_425340

Product name

CALR monoclonal antibody (M01), clone 1G11-1A9

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant CALR.

Antigen sequence

MLLSVPLLLGLLGLAVAEPAVYFKEQFLDGDGWTS
RWIESKHKSDFGKFVLSSGKFYGDEEKDKGLQTSQ
DARFYALSASFEPFSNKGQTLVVQFTVKHEQNIDC
GGGYVKLFPNSLDQTDMHGDSEYNIMFGPDICGPG
TKKVHVIFNYKGKNVLINKDIRCKDDEFTHLYTLI
VRPDNTYEVKIDNSQVESGSLEDDWDFLPPKKIKD
PDASKPEDWDERAKIDDPTDSKPEDWDKPEHIPDP
DAKKPEDWDEEMDGEWEPPVIQNPEYKGEWKPRQI
DNPDYKGTWIHPEIDNPEYSPDPSIYAYDNFGVLG
LDLWQVKSGTIFDNFLITNDEAYAEEFGNETWGVT
KAAEKQMKDKQDEEQRLKEEEEDKKRKEEEEAEDK
EDDEDKDEDEEDEEDKEEDEEEDVPGQAKDEL


Isotype

IgG

Antibody clone number

1G11-1A9

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

The angiogenesis inhibitor vasostatin is regulated by neutrophil elastase-dependent cleavage of calreticulin in AML patients.

Mans S, Banz Y, Mueller BU, Pabst T
Blood 2012 Sep 27;120(13):2690-9

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

CALR monoclonal antibody (M01), clone 1G11-1A9 Western Blot analysis of CALR expression in K-562 ( Cat # L009V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of CALR expression in transfected 293T cell line by CALR monoclonal antibody (M01), clone 1G11-1A9.Lane 1: CALR transfected lysate(48 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to CALR on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 1 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol