Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- LS-B14955 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ CALR / Calreticulin Antibody (aa 18-417) LS-B14955
- Antibody type
- Polyclonal
- Description
- Caprylic acid and ammonium sulfate precipitation
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of Calreticulin antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate . Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution . Predicted band size: 42 kDa . Observed band size: 70 kDa Additional bands at: 118 kDa. We are unsure as to the identity of these extra bands. This image was taken for the unconjugated form of this product. Other forms have not been tested.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, PC-3 whole cell lysate, Rat kidney tissue, Mouse kidney tissue All lanes: CALR antibody at 2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 49 kDa Observed band size: 49 kDa