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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- MAB10459 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB10459, RRID:AB_11183668
- Product name
- CALR monoclonal antibody, clone 1G6A7
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against synthetic peptide CALR.
- Antigen sequence
EEEDVPGQAKDELC- Isotype
- IgG
- Antibody clone number
- 1G6A7
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of CALR monoclonal antibody, clone 1G6A7 (Cat # MAB10459) against HeLa (1), A-549 (2), NTERA2 (3) and MCF-7 (4) cell lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using CALR monoclonal antibody, clone 1G6A7 (Cat # MAB10459) against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY CALR cDNA (2).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis of 3T3-L1 cells using CALR monoclonal antibody, clone 1G6A7 (Cat # MAB10459) (green). Blue: DRAQ5 fluorescent DNA dye.
- Validation comment
- Immunofluorescence
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis of SK-BR-3 (A) and A-549 (B) cells using CALR monoclonal antibody, clone 1G6A7 (Cat # MAB10459) (green).Red: Actin filaments have been labeled with DY-554 phalloidin.Blue: DRAQ5 fluorescent DNA dye.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human placenta tissue using CALR monoclonal antibody, clone 1G6A7 (Cat # MAB10459).
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
