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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunohistochemistry [1]
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- Product number
- A00894-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Calreticulin/CALR Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for Calreticulin/CALR detection. Tested with WB, IHC-P, FCM, Direct ELISA in Human; Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Label-free nonlinear optical signatures of extracellular vesicles in liquid and tissue biopsies of human breast cancer.
Calreticulin overexpression correlates with integrin-α5 and transforming growth factor-β1 expression in the atria of patients with rheumatic valvular disease and atrial fibrillation.
Sorrells JE, Park J, Aksamitiene E, Marjanovic M, Martin EM, Chaney EJ, Higham AM, Cradock KA, Liu ZG, Boppart SA
Scientific reports 2024 Mar 6;14(1):5528
Scientific reports 2024 Mar 6;14(1):5528
Calreticulin overexpression correlates with integrin-α5 and transforming growth factor-β1 expression in the atria of patients with rheumatic valvular disease and atrial fibrillation.
Zhao F, Zhang S, Shao Y, Wu Y, Qin J, Chen Y, Chen L, Gu H, Wang X, Huang C, Zhang W
International journal of cardiology 2013 Oct 3;168(3):2177-85
International journal of cardiology 2013 Oct 3;168(3):2177-85
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of CALR using anti-CALR antibody (A00894-1). CALR was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CALR Antibody (A00894-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image
