HPA002242

antibody from Atlas Antibodies

Targeting: CALR cC1qR, CRT, FLJ26680, RO, SSA

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA002242

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA002242, RRID:AB_1078384

Product name

Anti-CALR

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

EQNIDCGGGYVKLFPNSLDQTDMHGDSEYNIMFGP
DICGPGTKKVHVIFNYKGKNVLINKDIRCKDDEFT
HLYTLIVRPDNTYEVKIDNSQVESGSLEDDWDFLP
PKKIKDPDASKPEDWDERAKIDDPTDS


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line HL-60.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human thyroid gland and pancreas tissues using Anti-CALR antibody. Corresponding CALR RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human thyroid gland shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas shows low expression as expected.

Sample type

HUMAN