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LearnA301-778A
antibody from Invitrogen Antibodies
Targeting: NIPBL
DKFZp434L1319, FLJ11203, FLJ12597, FLJ13354, FLJ13648, IDN3, Scc2
Western blot
Immunoprecipitation
Other assayAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Other assay [1]
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- Product number
- A301-778A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NIPBL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Detection of human NIPBL by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NIPBL antibody A301-778A (lot A301-778A-2) used for IP at 6 µg per reaction. NIPBL was also immunoprecipitated by a previous lot of this antibody (lot A301-778A-1) and rabbit anti-NIPBL antibody A301-779A For blotting immunoprecipitated NIPBL, A301-779A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
