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LearnNB100-93320
antibody from Novus Biologicals
Targeting: NIPBL
DKFZp434L1319, FLJ11203, FLJ12597, FLJ13354, FLJ13648, IDN3, Scc2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [2]
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Validation data
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- Product number
- NB100-93320 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-93320, RRID:AB_1237230
- Product name
- Rabbit Polyclonal NIPBL Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references NIPBL: a new player in myeloid cell differentiation.
Mazzola M, Deflorian G, Pezzotta A, Ferrari L, Fazio G, Bresciani E, Saitta C, Ferrari L, Fumagalli M, Parma M, Marasca F, Bodega B, Riva P, Cotelli F, Biondi A, Marozzi A, Cazzaniga G, Pistocchi A
Haematologica 2019 Jul;104(7):1332-1341
Haematologica 2019 Jul;104(7):1332-1341
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NIPBL Antibody [NB100-93320] - Whole cell lysate (15 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NIPBL antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: NIPBL Antibody [NB100-93320] - Detection of Human NIPBL by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-NIPBL antibody NB100-93320 used for IP at 3 mcg/mg lysate. NIPBL was also immunoprecipitated by rabbit anti-NIPBL antibody NB100-93319, which recognizes an upstream epitope.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: NIPBL Antibody [NB100-93320] - Detection of human NIPBL by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NIPBL antibody NB100-93320 (lot 4) used for IP at 3 ug per reaction. NIPBL was also immunoprecipitated by a previous lot of this antibody (lot 3) and rabbit anti-NIPBL antibody NB100-93319. For blotting immunoprecipitated NIPBL, NB100-93320 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
