AP09208PU-N

antibody from Acris Antibodies GmbH

Targeting: NIPBL DKFZp434L1319, FLJ11203, FLJ12597, FLJ13354, FLJ13648, IDN3, Scc2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

AP09208PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP09208PU-N, RRID:AB_2035797

Product name

anti NIPBL (344-356)

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to amino acids 344-356 of Human IDN3

Reactivity

Human, Mouse, Rat, Bovine, Canine

Host

Goat

Isotype

IgG

Vial size

0.1 mg

Concentration

1.6 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using Affinity Purified anti-IDN3 antibody shows detection of bands at ~315 kDa and ~125 kDa corresponding to isoforms of IDN3 (arrowheads) in mouse heart whole cell tissue extract. Approximately 35 µg of lysate was separated on a 4-8% gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500. Reaction occurred overnight at 4° followed by washes and reaction with a 1:20,000 dilution of IRDye(TM)800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at room temperature. IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using Affinity Purified anti-IDN3 antibody shows detection of a band ~300 kDa corresponding to IDN3 (arrowhead) in human kidney (lane 1) and human heart (lane 2) whole cell tissue extracts. Approximately 20 µg of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:500. Use HRP conjugated Rb-a-Goat IgG [H&L] MXHu for detection.