A305-664A-T

antibody from Invitrogen Antibodies

Targeting: GLOD4 C17orf25, CGI-150, HC71

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A305-664A-T

Provider

Invitrogen Antibodies

Product name

GLOD4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

10 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse GLOD4 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, HeLa, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GLOD4 antibody (Product # A305-664A-T; lot A305-664A-T-2) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human GLOD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GLOD4 antibody A305-664A-M (lot A305-664A-M-1) used for IP at 20 µl per reaction. For blotting immunoprecipitated GLOD4, A305-664A-M was used at 1:100. Detection: Chemiluminescence with an exposure time of 10 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human GLOD4 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GLOD4 antibody A305-664A-M (lot A305-664A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human GLOD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GLOD4 antibody (Product # A305-664A-M (lot A305-664A-M-2) used for IP at 40 µL/mg lysate. GLOD4 was also immunoprecipitated by a previous lot of this antibody (lot A305-664A-T-1). For blotting immunoprecipitated GLOD4, Product # A305-664A-T was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.