HPA042250
antibody from Atlas Antibodies
Targeting: TADA3
ADA3, FLJ20221, FLJ21329, hADA3, NGG1, TADA3L
Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- HPA042250 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA042250, RRID:AB_10794043
- Product name
- Anti-TADA3
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human TADA3, Gene description: transcriptional adaptor 3, Alternative Gene Names: ADA3, FLJ20221, FLJ21329, hADA3, NGG1, TADA3L, Validated applications: ChIP, ICC, IHC, WB, Uniprot ID: O75528, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.2 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Epidermal Growth Factor Receptor activation promotes ADA3 acetylation through the AKT-p300 pathway
Alteration/Deficiency in Activation 3 (ADA3) Protein, a Cell Cycle Regulator, Associates with the Centromere through CENP-B and Regulates Chromosome Segregation
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Srivastava S, Mohibi S, Mirza S, Band H, Band V
Cell Cycle 2017;16(16):1515-1525
Cell Cycle 2017;16(16):1515-1525
Alteration/Deficiency in Activation 3 (ADA3) Protein, a Cell Cycle Regulator, Associates with the Centromere through CENP-B and Regulates Chromosome Segregation
Mohibi S, Srivastava S, Wang-France J, Mirza S, Zhao X, Band H, Band V
Journal of Biological Chemistry 2015;290(47):28299-28310
Journal of Biological Chemistry 2015;290(47):28299-28310
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C, Rexhepaj E, Singan V, Murphy R, Pepperkok R, Uhlén M, Simpson J, Lundberg E
Nature Methods 2013;10(4):315-323
Nature Methods 2013;10(4):315-323
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Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- ChIP-Exo-Seq composite graph for Anti-TADA3 (HPA042250, Lot 000010670) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.