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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Flow cytometry [1]
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Validation data
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- Product number
- GTX83953 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX83953, RRID:AB_10732156
- Product name
- OTUB2 antibody [11B3]
- Antibody type
- Monoclonal
- Reactivity
- Human, Rat, Canine, Simian
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-OTUB2 monoclonal antibody.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OTUB2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OTUB2.
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC209650 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-OTUB2 antibody(GTX83953), and then analyzed by flow cytometry.
