LS-C209623

antibody from LSBio

Targeting: ALDOA

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

LS-C209623

Provider

LSBio

Product name

ALDOA / Aldolase A Antibody (Lyophilized, HRP) LS-C209623

Antibody type

Polyclonal

Description

Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.

Reactivity

Human, Rabbit

Host

Goat

Conjugate

Horseradish Peroxidase

Isotype

IgG

Storage

Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IgG purified antibody to rabbit muscle aldolase was used at a 1:1000 dilution to detect human aldolase by western blot. A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% Tris glycine gradient gel for SDS-PAGE. The gel was transferred to nitro-cellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4°C in TTBS supplemented with 2% non-fat dry milk. Color was allowed to develop using SuperSignal West Pico Chemiluminescent Substrate (PIERCE). Other detection methods will yield similar results. This antibody clearly detects a band at ~41 kDa consistent with human aldolase. This image was taken for the unconjugated form of this product. Other forms have not been tested.